Protocols​

Below you find protocols that have been used in the GFN laboratory training courses. 

Additional protocols related to antimicrobial resistance, e.g. specific PCR-protocols (e.g. mcr-1, mcr-2, mecA, mecC), or protocols for isolation of ESBL-, AmpC-, and carbapenemase-producing E. coli from meat or caecal samples are available via the EURL-AR website (http://eurl-ar.eu/233-protocols.htm).

Antimicrobial susceptility testing​

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Salmonella /Shigella

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Salmonella/Shigella - Identification (biochemical)
Biochemical identification of Salmonella/Shigella using an abbreviated panel of tests - version 002, updated October 2015

(PDF document, 800 KB)

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Salmonella - Identification (API)
(PDF document, 605 KB)

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Salmonella - Serotyping
(PDF document, 339 KB)

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Shigella - Serotyping
(PDF document, 715 KB)

Campylobacter​

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Campylobacter - Isolation (faeces)

(PDF docukent, 332 KB)

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Campylobacter - Isolation (food)
(PDF document, 329 KB)

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Campylobacter - Isolation (water)
(PDF document, 341 KB)

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Campylobacter - Identification
(PDF document, 566 KB)

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Campylobacter - Identification (PCR)

(PDF document, 140 KB)

E. coli 0157​

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E. coli - Agglutination
(PDF document, 390 KB)

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E. coli - Agglutination (Latex)
(PDF document, 394 KB)

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E. coli - Identification (PCR)
(PDF document, 732 KB)

MLVA​

​A protocol on MLVA of Salmonella enterica serotype Typhimurium is available on the ECDC website: http://ecdc.europa.eu/en/publications/Publications/Forms/ECDC_DispForm.aspx?ID=748

Pathogenic E. coli​

PFGE​

Protocols on Pulsed Field Gel Electroforesis are available on the PulseNet International website: http://www.pulsenetinternational.org/protocols/Pages/default.aspx

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INFORMATION​

Technical University of Denmark, National Food Institute

CVR: 30060946

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E-mail: suska@food.dtu.dk