Protocols​

Below you find protocols that have been used in the GFN laboratory training courses. 

Additional protocols related to antimicrobial resistance, e.g. specific PCR-protocols (e.g. mcr-1, mcr-2, mecA, mecC), or protocols for isolation of ESBL-, AmpC-, and carbapenemase-producing E. coli from meat or caecal samples are available via the EURL-AR website (http://eurl-ar.eu/233-protocols.htm).

Antimicrobial susceptility testing​

pdfikon

Salmonella /Shigella

pdfikon

Salmonella/Shigella - Identification (biochemical)
Biochemical identification of Salmonella/Shigella using an abbreviated panel of tests - version 002, updated October 2015

(PDF document, 800 KB)

pdfikon

Salmonella - Identification (API)
(PDF document, 605 KB)

pdfikon

Salmonella - Serotyping
(PDF document, 339 KB)

pdfikon

Shigella - Serotyping
(PDF document, 715 KB)

Campylobacter​

pdfikon

Campylobacter - Isolation (faeces)

(PDF docukent, 332 KB)

pdfikon

Campylobacter - Isolation (food)
(PDF document, 329 KB)

pdfikon

Campylobacter - Isolation (water)
(PDF document, 341 KB)

pdfikon

Campylobacter - Identification
(PDF document, 566 KB)

pdfikon

Campylobacter - Identification (PCR)

(PDF document, 140 KB)

E. coli 0157​

pdfikon

pdfikon

E. coli - Agglutination
(PDF document, 390 KB)

pdfikon

E. coli - Agglutination (Latex)
(PDF document, 394 KB)

pdfikon

E. coli - Identification (PCR)
(PDF document, 732 KB)

MLVA​

​A protocol on MLVA of Salmonella enterica serotype Typhimurium is available on the ECDC website: http://ecdc.europa.eu/en/publications/Publications/Forms/ECDC_DispForm.aspx?ID=748

Pathogenic E. coli​

PFGE​

Protocols on Pulsed Field Gel Electroforesis are available on the PulseNet International website: https://www.cdc.gov/pulsenet/pathogens/index.html

Disclaimer​

​Information presented on this website is considered public information (unless otherwise noted) and may be distributed or copied. Use of appropriate byline/photo/image credit is requested. While The National Food Institute makes every effort to provide accurate and complete information, various data such as names, telephone numbers, etc. may change prior to updating. The National Food Institute welcomes suggestions on how to improve our home page and correct errors. The National Food Institute provides no warranty, expressed or implied, as to the accuracy, reliability or completeness of furnished data.

Some of the documents on this server may contain live references (or pointers) to information created and maintained by other organizations. Please note that The National Food Institute does not control and cannot guarantee the relevance, timeliness, or accuracy of these outside materials.

INFORMATION​

Technical University of Denmark, National Food Institute

CVR: 30060946

AdDress

Denmark

DK-2800 Lyngby

Kemitorvet Building 204

Contact us

Tlf.: +45 3588 6601

E-mail: suska@food.dtu.dk