External quality assessment (EQA) for laboratories participating in the European Antimicrobial Resistance Surveillance Network (EARS-Net)​

The European Antimicrobial Resistance Surveillance Network (EARS-Net), formerly known as the European Antimicrobial Resistance Surveillance System (EARSS), provides analyses of trends in antimicrobial susceptibility over time and between all EU Member states and two EEA countries (Iceland and Norway). Data are based on routine antimicrobial susceptibility test results collected from a network of clinical laboratories. At present, the pathogens included in the surveillance network are Streptococcus pneumoniae, Staphylococcus aureus, Enterococcus faecalis, Enterococcus faecium, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Acinetobacter spp.

EARS-Net is administrated and coordinated by the European Centre for Disease Prevention and Control (ECDC).

Since 2000, EARSS/EARS-Net has organised External Quality Assessment (EQA) exercises in antimicrobial susceptibility testing across clinical EARS-Net participating microbiology laboratories in EU/EEA countries.​

The EQA exercises provide important information on performance and comparability of the reported test results between participating laboratories and countries.

The objectives of the EARS-Net EQA exercises are:

  • ​​To assess the quality of the species identification;
  • To assess the accuracy of qualitative antimicrobial susceptibility test results reported by participating individual laboratories;​

  • To evaluate the overall comparability of routinely collected test results between laboratories and European Union/European Economic Area (EU/EEA) countries. 

Participation in EQA exercises is useful for production of reliable laboratory results of consistently good quality. In the EARS-Net EQA exercises, laboratories are requested to test the bacterial strains by the methods routinely used and the results should then be submitted through a web tool (password-protected) which will generate an evaluation report after validation of the submitted results. 

Further, a national summary report will be shared with the National EARS-Net EQA Coordinator. The report will provide a summary of results for their country, including a short conclusion on the capacity of the participating national laboratories, and if applicable, recommendations for improvement.

​An EU/EEA summary report (anonymised) will be published on the ECDC website.

Since 2021, the EARS-Net EQA exercises have been organised by ECDC in collaboration with DTU Food.

For your information: ECDC has decided to include species relevant for the EARS-Net surveillance for species identification. Thus, the six strains may be one of the eight species included in the EARS-Net surveillance or a species not part of the EARS-Net surveillance. In the protocol it is described how to handle the different strains.

For further information, please contact Birgitte Helwigh earsnet-eqa@food.dtu.dk

​Documents for the 2024 EARS-Net EQA


2024 EARS-Net EQA webtool guideline to upload get access to the platform for upload results

2024 EARS-Net EQA Protocol contains:

o laboratory handling of the bacterial cultures;
o the tests included in this EQA exercise;
o instructions on how to report EQA results using the webtool and;
o information about the scoring system:

Test forms 

We recommend using the test forms to record the obtained EQA results before entering the results into the webtool.

EARS-Net EQA 2024 test form Acinetobacter baumannii

EARS-Net EQA 2024 test form Enterococcus faecalis

EARS-Net EQA 2024 test form Enterococcus faecium

EARS-Net EQA 2024 test form Escherichia coli

EARS-Net EQA 2024 test form Klebsiella pneumoniae

EARS-Net EQA 2024 test form Pseudomonas aeruginosa

EARS-Net EQA 2024 test form Staphylococcus aureus

EARS-Net EQA 2024 test form Streptococcus pneumoniae

Documents for the 2023 EARS-Net EQA

Expected results for the 2023 EARS-Net EQA

​​2023 EARS-Net EQA protocol

Documents for the 2022 EARS-Net EQA

2022 EARS-Net EQA summary report

2022 EARS-Net EQA protocol

Documents for 2021 EARS-Net EQA

2021 EARS-Net EQA summary report

2021 E​ARS-Net EQA protocol

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