Protocols

Below you find protocols that have been used in the GFN laboratory training courses.

Additional protocols related to antimicrobial resistance, e.g. specific PCR-protocols (e.g. mcr-1, mcr-2, mecA, mecC), or protocols for isolation of ESBL-, AmpC-, and carbapenemase-producing E. coli from meat or caecal samples are available via the EURL-AMR website Protocols: https://www.food.dtu.dk/english/topics/antimicrobial-resistance/eurl-ar/protocols

Other resources: https://www.food.dtu.dk/english/topics/antimicrobial-resistance/eurl-ar/resources

Antimicrobial susceptility testing

Susceptibility Testing of Enterobacteriaceae using Disc Diffusion

(PDF document, 190 KB)

Antimicrobial Susceptibility Testing - E-test
(PDF document, 679 KB)

Antimicrobial Susceptibility Testing - Microbroth Dilution
(PDF document, 400 KB)

Antimicrobial Susceptibility Testing - Agar Dilution
(PDF document, 1,39 MB)

Salmonella /Shigella

Isolation of Salmonella spp. from Food and Animal Faeces
(PDF document 320 KB)

Isolation of Salmonella and Shigella from Faecal Specimens

PDF document, 500 KB

Salmonella/Shigella - Identification (biochemical)
Biochemical identification of Salmonella/Shigella using an abbreviated panel of tests - version 002, updated October 2015

(PDF document, 800 KB)

Salmonella - Identification (API)
(PDF document, 605 KB)